Endotoxin testing has been one of the most commonly requested needs among nanotech researchers applying to the NCL. Dr. Marina Dobrovolskaia, Sr. Principal Scientist, has spent more than a decade studying the intricacies of endotoxin detection in nanoformulations. Much of her work has focused on assessing the pros and cons of the various detection methods and determining ways to overcome common interference issues seen with nanoformulations. Alongside Marina, Barry Neun (Research Associate III) has been NCL’s technical expert in conducting the often-requested Limulus Amoebocyte Lysate (LAL) assay.
Each year, Marina and Barry advise dozens of researchers on the complexities of the LAL assays through seminars, publications, personalized consultations, and training sessions. Recently, they supplemented these efforts by publishing a video demonstration of the LAL assay to support the written protocols. Published in the Journal of Visualized Experiments, the video outlines the procedure for the turbidity and gel-clot LAL assays, including preparation of the standard curve, quality controls, instrumentation settings, and more.
View and download the Journal of Visualized Experiments protocol here.
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
Barry W. Neun and Marina A. Dobrovolskaia
Journal of Visualized Experiments, 2019, 143, e58830, doi:10.3791/58830.
You can also find several related resources on our website:
- Protocols on endotoxin testing, including end-point chromogenic, kinetic chromogenic, kinetic turbidity, and gel-clot LAL assays: https://ncl.cancer.gov/resources/assay-cascade-protocols
- Manuscripts on endotoxin testing, including selection of method, common interferences, and minimizing contamination. These citations can be found on our bibliography page.